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- Title
N-glycosylation of the human melanocortin 1 receptor: occupancy of glycosylation sequons and functional role.
- Authors
Herraiz, Cecilia; Sánchez-Laorden, Berta L.; Jiménez-Cervantes, Celia; García-Borrón, José C.
- Abstract
The melanocortin 1 receptor (MC1R), a major determinant of skin pigmentation and phototype, mediates the actions of α-melanocyte-stimulating hormone on melanocytes and is critical for melanocyte proliferation and differentiation. MC1R has two putative N-glycosylation targets, Asn15 and Asn29. It has been shown that MC1R is a glycoprotein with an unusual sensitivity to endoglycosidase H digestion. However, the occupancy and functional importance of each specific glycosylation sequon remains unknown. We demonstrate that MC1R is N-glycosylated at Asn15 and Asn29, with structurally and functionally different glycan chains. N-glycosylation is not necessary for high affinity agonist binding or functional coupling but has a strong effect on the availability of MC1R molecules on the plasma membrane, most likely by a combination of improved forward trafficking and decreased internalization. Finally, we found that MC1R variants exhibit different degrees of glycosylation which do not show a simple correlation with their functional status or intracellular trafficking.
- Subjects
GLYCOSYLATION; HUMAN skin color; MELANOCYTES; ENDOGLYCOSIDASES; GLYCOPROTEINS
- Publication
Pigment Cell & Melanoma Research, 2011, Vol 24, Issue 3, p479
- ISSN
1755-1471
- Publication type
Article
- DOI
10.1111/j.1755-148X.2011.00848.x