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- Title
Tautomers of a Fluorescent G Surrogate and Their Distinct Photophysics Provide Additional Information Channels.
- Authors
Sholokh, Marianna; Improta, Roberto; Mori, Mattia; Sharma, Rajhans; Kenfack, Cyril; Dongwon Shin; Voltz, Karine; Stote, Roland H.; Zaporozhets, Olga A.; Botta, Maurizio; Tor, Yitzhak; Mély, Yves
- Abstract
Thienoguanosine (thG) is an isomorphic nucleoside analogue acting as a faithful fluorescent substitute of thG, with respectable quantum yield in oligonucleotides. Photophysical analysis of thG reveals the existence of two ground-state tautomers with significantly shifted absorption and emission wavelengths, and high quantum yield in buffer. Using (TD)- DFT calculations, the tautomers were identified as the H1 and H3 keto-amino tautomers. When incorporated into the loop of (-)PBS, the (-)DNA copy of the HIV-1 primer binding site, both tautomers are observed and show differential sensitivity to protein binding. The red-shifted H1 tautomer is strongly favored in matched (-)/(+)PBS duplexes, while the relative emission of the H3 tautomer can be used to detect single nucleotide polymorphisms. These tautomers and their distinct environmental sensitivity provide unprecedented information channels for analyzing G residues in oligonucleotides and their complexes.
- Subjects
TAUTOMERISM; STRUCTURAL isomerism; GUANOSINE derivatives; NUCLEOSIDE derivatives; INSTITUTIONAL isomorphism
- Publication
Angewandte Chemie International Edition, 2016, Vol 55, Issue 28, p7974
- ISSN
1433-7851
- Publication type
Article
- DOI
10.1002/anie.201601688