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- Title
The biological effect of minocycline on human periodontal ligament fibroblast.
- Authors
Wang Yi; Liu Hong-chen; E. Ling-ling; Li Shi-jun; Zhao Zheng; Wang Dong-sheng
- Abstract
PURPOSE: To investigate the effective concentration range of minocycline as a local drug delivery system. METHODS: A pilot study of minocycline on proliferation, protein synthesis, collagen I synthesis, ALP activity, osteocalcin secretion of human periodontal ligament fibroblast (hPDLF) was carried out. Different concentrations of minocycline (0, 10, 20, 40, 100, 200mg/L) were added to the fifTh passage (P5) hPDLF. After 1, 4, 7, 10, 14 days of co-incubation, the proliferation activity was measured with MTT assay; Protein synthesis, collagen I synthesis, ALP activity and osteocalcin secretion were measured. Statistical analysis was performaed with SPSS 13.0 software package. RESULTS: 10-200mg/L minocycline could significantly increase the proliferation of PS hPDLF (P<0.05), and 100mg/L minocycline was the optimal effect. 20-200mg/L minocycline could significantly increase protein synthesis and collagen I synthesis (P<0.05); 10mg/L. could significantly increase ALP activity (P<0.05), 20-200mg/L could significantly inhibit ALP activity (P<0.05); 40-200mg/L could significantly inhibit osteocalcin secrction (P<0.05). 10-20mg/L minocycline increased proliferation, protein synthesis, collagen I synthesis. ALP activity, osteocalcin secretion of P5 hPDLF, but did not inhibit osteocalcin secretion. CONCLUSION: 10-20mg/L might be the effective concentration range of minocycline as a local drug delivery system. Supported by National Natural Science Foundation of China(Grant No.30973354)
- Subjects
DRUG delivery systems; TETRACYCLINES; ANTIBIOTICS; PERIODONTAL ligament; FIBROBLASTS; DRUG efficacy
- Publication
Shanghai Journal of Stomatology, 2010, Vol 19, Issue 2, p162
- ISSN
1006-7248
- Publication type
Article