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- Title
Effects of glucose and its modulation by insulin on proliferation and differentiation of rat dental follicle cells in vitro.
- Authors
LIU Hong-chen; XU Lu-lu; WANG Dong-sheng; Jiao, L. V.
- Abstract
PURPOSE: To investigate the effects of glucose and its modulation by insulin on proliferation and differentiation of rat dental follicle cells (DFCs) in vitro. METHODS: The rat dental follicle cells from 7-day neonatal rat were cultured. The effects of different doses of glucose concentration (5.5, 16.5, and 49.5mmol/L), alone with insulin (6µg/ml), on rat dental follicle cells was examined in vitro. The DFCs proliferation and alkaline phosphatase (ALP) were examined after 1,3,7,9 days and 1-7 days of culture, respectively. The data were analyzed using SPSS13.0 software package. RESULTS: At 1,3 day, DFCs proliferation greatly enhanced at 16.5mmol/L glucose, and decreased at 49.5mmol/L glucose. Insulin treatment greatly decreased the proliferation of 16.5mmol/L glucose and increased the proliferation of 49.5mmol/L glucose. With high concentrations of glucose, cell proliferation greatly increased at 7,9 day, insulin treatment greatly increased the proliferation of DFCs. High concentrations of glucose greatly increased the DFCs ALP activity in 1-7 days. Insulin treatment greatly decreased DFCs ALP activity with high concentrations of glucose (P<0.05). CONCLUSION: This study suggests that insulin contains the deleterious effect of acute high concentrations of glucose on rat DFCs proliferation and differentiation.
- Subjects
BLOOD sugar; INSULIN; CELL proliferation; CELL differentiation; TEETH
- Publication
Shanghai Journal of Stomatology, 2008, Vol 17, Issue 4, p425
- ISSN
1006-7248
- Publication type
Article