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- Title
Role of calmodulin and myosin light chain kinase in the activation of carbachol-activated cationic current in murine ileal myocytes.
- Authors
Jeon, Ju-Hong; Kim, Seon Jeong; So, Insuk; Kim, Byung Joo
- Abstract
We investigated the effect of calmodulin (CaM) and myosin light chain kinase (MLCK) on murine ileal myocytes using the whole-cell patch-clamp technique. Under the voltage clamp, at the holding potential of –60 mV, 50 μmol/L carbachol (CCh) induced inward currents (ICCh), and spontaneous decay of ICCh occurred. The peak inward currents induced by the repetitive application of CCh (50 μmol/L) tended to decrease in amplitude. Intracellular application of 0.2 mmol/L guanosine 5′-O-(γ-thio)triphosphate (GTPγS) from the patch electrode induced an inward current at a holding potential of –60mV, and the peak inward currents induced by the repetitive application of Cs tended to decrease slightly in amplitude. The amplitude of ICCh was reduced by pretreatment either with W-7, trifluoroperazine, W-5, and melittin (CaM inhibitors) or with ML-7 and ML-9 (selective MLCK inhibitors), and the inhibitory effects were reversible. However, when we pretreated with 50 μmol/L W-7 or 5 μmol/L ML-7 on GTPγS-induced inward currents, almost no inhibition was observed in the inward currents. Application of both Rho kinase inhibitor and MLCK inhibitor inhibited GTPγS-induced currents. We conclude that CaM and MLCK modulate the activation process of ICCh in murine ileal myocytes and suggest that the classical type transient receptor potential (TRPC) channel 5 might be a candidate for nonselective cationic currents (NSCC) activated by muscarinic stimulation in gastrointestinal smooth muscle cells.
- Subjects
CALMODULIN; MYOSIN; MUSCLE cells; VOLTAGE-clamp techniques (Electrophysiology); CELLS
- Publication
Canadian Journal of Physiology & Pharmacology, 2007, Vol 85, Issue 12, p1254
- ISSN
0008-4212
- Publication type
Article
- DOI
10.1139/Y07-118