We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Signalling via MHC Class II Molecules Modifies the Composition of GEMs in APC.
- Authors
Setterblad, N.; Becart, S.; Charron, D.; Mooney, N.
- Abstract
Major histocompatibility complex (MHC) class II molecules are responsible for peptide presentation to helper T lymphocytes and as such play an essential role in the immune response. These molecules transmit intracellular signals leading to diverse consequences in B lymphocytes including proliferation and apoptosis. Recent studies have revealed that glycolipid enriched membrane microdomains (GEMs) behave as signalling platforms for a variety of lymphocyte receptors. We have quantified human leucocyte antigen (HLA)-DR molecules localized in GEMs in human B lymphocytes. Use of a model imitating the interaction of HLA-DR with a T-cell receptor (TCR) modified the constituents of the HLA–DR-enriched GEMs. Confocal microscopy demonstrated a recruitment of HLA–DR and the ganglioside GM1 at the site of HLA–DR interaction with the stimulating ligand. Moreover, cholesterol depletion efficiently impaired this recruitment. Co-localizing proteins detected in HLA–DR-enriched GEMs include protein kinase C (PKC)-δ and actin. These data reveal that MHC class II antigens are localized in GEMs in mature human B lymphocytes and indicates that the formation of the immunological synapse regulates the composition of HLA–DR enriched GEMs in the antigen presenting cell (APC).
- Subjects
MAJOR histocompatibility complex; T cells; GLYCOLIPIDS; LYMPHOCYTE receptors
- Publication
Scandinavian Journal of Immunology, 2001, Vol 54, Issue 1/2, p87
- ISSN
0300-9475
- Publication type
Article
- DOI
10.1046/j.1365-3083.2001.00969.x