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- Title
Tumour Necrosis Factor-&chl; Induces Neutrophil-Mediated Injury of Cultured Human Endothelial Cells.
- Authors
Von Asmuth, E. J. U.; Leeuwenberg, J. F. M.; Van Der Linden, C. J.; Buurman, W. A.
- Abstract
We investigated the ability of TNF-α to mediate damage of endothelial cells in the presence of Neutrophils, by measuring detachment of cultured human umbilical vein endothelial cells (HUVEC), Endothelial cell detachment was increased from 5% to about 75% by the presence of 1-10 ng/ml TNF-α during incubation with neutrophils, whereas negligible endothelial cell lysis was observed as measured by 51Cr release, TNF-α was compared with the cytokines IL-lα and IFN-γ and with PMA and LPS, Both TNF-α and PMA appeared to be strong triggers for neutrophil-induced endothelial cell detachment, whilst reduced injury was seen after addition of IL-α and LPS, IFN-γ did not induce endothelial cell detachment, but potentiated the effect of both TNF-α and IL-α, TNF-α-induced endothelial cell detachment was neutrophil dependent, since pre-incubation of neutrophils, but not pre-incubation of endothelial cells with TNF-α, caused endothelial cell detachment. Thus, TNF-α-induced increase in neutrophil-adhesiveness of HUVEC was found not to be essential for endothelial damage, Pre-incubation of neutrophils in suspension with TNF-α induced rapid activation, followed by nearly complete deactivation of neutrophils, as measured by their capacity to induce detachment of endothelial cells after removal of TNF-α. These results indicate that local presence of TNF-a might be critical in tissue or organ damage during early, neutrophil-mediated inflammatory processes, independent of enhanced adhesiveness of endothelium for neutrophils.
- Subjects
NEUTROPHILS; GRANULOCYTES; CYTOKINES; GROWTH factors; TUMOR necrosis factors; BLOOD vessels
- Publication
Scandinavian Journal of Immunology, 1991, Vol 34, Issue 2, p197
- ISSN
0300-9475
- Publication type
Article
- DOI
10.1111/j.1365-3083.1991.tb01537.x