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- Title
Positive Selection of Antigen-Specific B Lymphocytes by Means of Immunomagnetic Particles.
- Authors
Elegand, T.; Hovdenes, A.; Lea, T.
- Abstract
This paper presents a method for the isolation of highly enriched antigen-specific B lymphocytes from peripheral blood mononuclear cells (PBMC) by means of an immunomagnetic procedure. The method is illustrated by the isolation of B cells positive for rheumatoid factors (RF) from patients with rheumatoid arthritis and healthy blood donors. After Epstein-Barr virus transformation of isolated cells, more than 90% of the generated immunoglobulin-secreting cells produced RF in several experiments. Compared to the low frequency of B cells positive for RF in PBMC, this represents a 10³- to 104-fold enrichment of antigen-specific B cells.
- Subjects
LYMPHOCYTES; B cells; RHEUMATOID factor; RHEUMATOID arthritis; ANTI-immunoglobulin autoantibodies; IMMUNOGLOBULINS; ANTIGEN-antibody reactions
- Publication
Scandinavian Journal of Immunology, 1988, Vol 27, Issue 4, p439
- ISSN
0300-9475
- Publication type
Article