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- Title
IgG- and IgM-Induced Cellular Cytotoxicity.
- Authors
Zöller, M.; Heyman, B.; Andrightetto, G.; Wigzell, H.
- Abstract
The actual existence of IgM-induced antibody-dependent cell-mediated cytolysis (ADCC) is still a matter of debate. In the present study four IgM anti-sheep erythrocyte (SRBC) and one IgM anti-trinitrophenyl (TNP) monoclonal hybridomas have been studied for their ability lo produce antibodies with ADCC-inducing capacity. All live hybridomas were shown to release into the culture supernatant antibodies with specific ADCC-inducing capacity. Compared with IgG-induced ADCC, however, much higher concentrations of IgM antibodies were necessary. To exclude the possibility that low numbers of IgG antibodies could be present in the culture supernatants, derived from hypothetical switching of hybridoma cells from IgM to IgG, several sets of controls were performed. Positioning of the inducing antibody with regard to size agreed with it being of IgM nature. Passage through protein A (PA) Sepharose columns removed no ADCC activity from the IgM hybridoma supernatants while completely retaining the activity from IgG hybridoma supernatant. In contrast, after passage over a concanavalin A (Con A)-Sepharose column. ADCC activity from IgM hybridoma supernatants was recovered in the bound and eluted fraction. Addition of aggregated human gammaglobulin (aggHGG) caused a complete inhibition of IgG-induced ADCC but did not significantly inhibit IgM-induced ADCC. Finally, trypsin treatment of the effector cells removed their ability to function in IgM ADCC, but IgG ADCC was left largely intact. We conclude from the above findings that IgM antibodies can function in ADCC in the absence of IgG antibodies, although their efficiency on a molar basis is significantly inferior to that of IgG antibodies.
- Subjects
IMMUNOGLOBULIN G; IMMUNOGLOBULINS; ANTINEOPLASTIC antibiotics; ERYTHROCYTES; CELLULAR pathology; THERAPEUTICS
- Publication
Scandinavian Journal of Immunology, 1982, Vol 16, Issue 5, p379
- ISSN
0300-9475
- Publication type
Article
- DOI
10.1111/j.1365-3083.1982.tb00738.x