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- Title
Cross-reacting Xenoantibodies to the Heavy Chain of H-2 and HLA-A,B Antigens: Serologic and Immunochemical Characterization.
- Authors
Callahan, G. N.; Pellegrino, M. A.; Ferrone, S.
- Abstract
H-2 cross-reactive antibodies present in HLA xenoantisera were purified by absorption on and elution from murine cells. Antibodies isolated from one of these antisera, 78-E48, were found to mediate complement-dependent lysis of both human and murine lymphoid cells but not of Daudi cells or of human lymphoid cells coated with Fab2 fragments from a cow anti-human β2-microglobulin (β2m) antiserum. In indirect immunoprecipitation analyses 78-E48 reacted only with proteins of approximately 45,000 and 12,000 MW present in NP-40 extracts of both human and murine lymphoid cells. Sequential precipitation experiments with rabbit anti-human β2m and allo-anti-H-2Kk, lak sera established that these proteins were in fact H-2 and HLA-A,B antigens. It was also found that 78-E48 reacted only with the heavy chain of HLA-A,B and H-2 antigens, since this eluate was unreactive with β2m in a radioimmunoassay, and its capacity to immunoprecipitate the 45,0000 and 12,000 MW proteins from human cell extracts was unaffected by prior reaction with purified human β2m. These data show for the first time that H-2 and HLA-A,B antigens share properties that probably depend upon their tertiary structure.
- Subjects
IMMUNOGLOBULINS; ANTIGENS; IMMUNITY; IMMUNE serums; SERUM; BLOOD plasma
- Publication
Scandinavian Journal of Immunology, 1980, Vol 12, Issue 4, p297
- ISSN
0300-9475
- Publication type
Article
- DOI
10.1111/j.1365-3083.1980.tb00070.x