We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Levan-type fructooligosaccharide production using Bacillus licheniformis RN-01 levansucrase Y246S immobilized on chitosan beads.
- Authors
Sangmanee, Surawut; Nakapong, Santhana; Pichyangkura, Rath; Kuttiyawong, Kamontip
- Abstract
Bacillus licheniformis RN-01 levansucrase Y246S (LsRN-Y246S) was immobilized by covalently linking onto chitosan, Sepabead EC-EP, and Sepabead EC-HFA, beads. The stability of immobilized LsRN-Y246S was found to be the highest with chitosan beads, retaining more than 70% activity after 13 weeks storage at 4 oC, and 68% activity after 12 hours incubation at 40°C. LsRN-Y246S immobilized on chitosan beads withstands sucrose concentrations up to 70% (w/v), retaining over 85% of its activity, significantly better than LsRN-Y246S immobilized on others supporting matrices. LsRN-Y246S immobilized on chitosan showed a 2.4 fold increase in activity in the presence of Mn2+, and gave slight protection against deactivation by of Cu2+, Zn2+, Fe3+, SDS and EDTA. A maximum of 8.36 g and an average of 7.35 g LFOS yield at least up to DP 11 can be produced from 25 g of sucrose, during five production cycles. We have demonstrated that LFOS can be effectively produced by chitosan immobilized LsRN-Y246S and purified.
- Subjects
FRUCTOOLIGOSACCHARIDES; BACILLUS licheniformis; LEVANSUCRASE; CHITOSAN; THERAPEUTIC immobilization
- Publication
Songklanakarin Journal of Science & Technology, 2016, Vol 38, Issue 3, p295
- ISSN
0125-3395
- Publication type
Article