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- Title
Characterization of a heat-activated retrotransposon in Vigna angularis.
- Authors
Yukari Masuta; Akira Kawabe; Kosuke Nozawa; Ken Naito; Atsushi Kato; Hidetaka Ito
- Abstract
In plants, several transposable elements are conserved across species. We found a homolog of ONSEN, which is a heat-activated retrotransposon originally isolated from Arabidopsis thaliana, in Vigna. The ONSEN-like elements (VaONS) were detected in all the analyzed Japanese accessions of Vigna angularis (adzuki bean) by Southern blot analysis. However, VaONS sequences were observed to be polymorphic in the different accessions. Interestingly, extrachromosomal DNA (ecDNA) was detected in some accessions of adzuki bean, indicating the conserved heat-activation of VaONS. Furthermore, we successfully induced retrotransposition of VaONS in adzuki plant regenerated through callus. Findings of our study should provide a new tool for molecular breeding of adzuki bean.
- Subjects
RETROTRANSPOSONS; AZUKI; EFFECT of heat on plants; EXTRACHROMOSOMAL DNA; CALLUS (Botany)
- Publication
Breeding Science, 2018, Vol 68, Issue 2, p168
- ISSN
1344-7610
- Publication type
Article
- DOI
10.1270/jsbbs.17085