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- Title
A combination of methods to evaluate biofilm production may help to determine the clinical relevance of Staphylococcus in blood cultures.
- Authors
Iorio, Natalia Lopes Pontes; Lopes, Ana Paula da Cruz Neves; Schuenck, Ricardo Pinto; Barcellos, Ariane Guimarães; Olendzki, André Nogueira; Lopez, Guillermo Lopez; dos Santos, Kátia Regina Netto
- Abstract
Staphylococcus is the most prevalent pathogen causing bacteremia and many of its isolates possess the ability to form biofilm. In this study Staphylococcus isolates from the blood of patients with bacteremia were analyzed by two biofilm detection phenotypic methods: Congo red agar (CRA) and microtiter-plate adherence (MPA) in relation to the presence of ica genes, detected by PCR. Their oxacillin susceptibility was also evaluated. Among 127 isolates evaluated, 47 were S. aureus and 80 were coagulase negative staphylococci (CNS). Seventy-four (58.3%) isolates were mecA gene positive (27.7% S. aureus and 76.3% CNS isolates). Among the 40 S. aureus isolates which were positive for the ica genes, 25 (62.5%) were positive in MPA and 27 (67.5%) in CRA, whereas both methods combined detected 34 (85%) isolates as biofilm producers. Among 12 S. epidermidis isolates carrying ica genes, 8 were positive in MPA and 5 in CRA. The combination of CRA and MPA methods provided a better prediction of the presence of ica genes in S. aureus isolates than did either method alone.
- Subjects
STAPHYLOCOCCUS; DETECTION of microorganisms; BACTEREMIA; BLOOD diseases; POLYMERASE chain reaction; BIOFILMS; OXACILLIN; MICROBIAL exopolysaccharides
- Publication
Microbiology & Immunology, 2011, Vol 55, Issue 1, p28
- ISSN
0385-5600
- Publication type
Article
- DOI
10.1111/j.1348-0421.2010.00288.x