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- Title
Identification and determination of ergot alkaloids in Morning Glory cultivars.
- Authors
Nowak, Julia; Woźniakiewicz, Michał; Klepacki, Piotr; Sowa, Anna; Kościelniak, Paweł
- Abstract
Seeds of plants from Ipomoea genera contain numerous ergot alkaloids, including psychoactive ergine and ergometrine, and are often abused as so-called 'legal highs.' In this work, an analytical method for determination of ergine and ergometrine, and identification of other alkaloids was developed, optimized, and validated. Three extraction techniques, ultrasound-assisted extraction in bath, or with sonotrode, and microwave-assisted extraction were evaluated, and it was concluded that ultrasonic bath is the most suitable technique for extraction of ergot alkaloids. The extraction method was later optimized using a Doehlert experimental design with response surface methodology and used together with the optimized LC-Q-TOF-MS method. The analytical procedure was validated in terms of recovery and matrix effect, repeatability, and intermediate precision. Limits of detection and quantification were 1.0 and 3.0 ng mL, respectively, and were sufficient for determination of ergot alkaloids in Ipomoea seeds. The analysis revealed that from five kinds of seeds purchased from different vendors, only three contained ergot alkaloids. Concentration of alkaloids and their relative abundance was similar in samples representative for whole seeds packs; however, when single seeds were analyzed, significant discrepancies in ergine and ergometrine concentrations were detected. [Figure not available: see fulltext.]
- Subjects
ERGOT alkaloids; ADRENERGIC alpha blockers; IDENTIFICATION; ERGOMETRINE; EXTRACTION techniques; EXPERIMENTAL design
- Publication
Analytical & Bioanalytical Chemistry, 2016, Vol 408, Issue 12, p3093
- ISSN
1618-2642
- Publication type
Article
- DOI
10.1007/s00216-016-9322-5