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- Title
Functional evaluation of hemodynamic response during neural activation using optical microangiography integrated with dual-wavelength laser speckle imaging.
- Authors
Jia Qin; Lei Shi; Hequn Wang; Reif, Roberto; Ruikang K. Wang
- Abstract
Evaluation of spatiotemporal hemodynamic and metabolic responses during neural activation is crucial in studying brain function. We explore the use of a noninvasive multifunctional optical imaging system to measure these responses in a mouse brain upon electrically stimulated neural activation, with the cranium left intact. The system is developed by integrating an optical microangiography (OMAG) imaging system with a dual-wavelength laser speckle imaging (DW-LSI) system. The DW-LSI, running at an image acquisition speed of ~100 Hz, is used to extract the large-scale two-dimensional map, revealing the localized response of blood flow, hemoglobin concentration, and metabolic rate of oxygen change. Guided by DW-LSI, the OMAG is, however, used to image the response of individual blood vessels with its unique depth-resolved capability. We show that the integrated system is capable of investigating neural activation, thus is potentially valuable in the preclinical study of the mechanism of neurovascular coupling.
- Subjects
HEMODYNAMICS; BRAIN function localization; LABORATORY mice; MEDICAL imaging systems; WAVELENGTHS; BLOOD vessels
- Publication
Journal of Biomedical Optics, 2014, Vol 19, Issue 2, p1
- ISSN
1083-3668
- Publication type
Article
- DOI
10.1117/1.JBO.19.2.026013