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- Title
Expression profiling of a complex thaumatin-like protein family in western white pine.
- Authors
Jun-Jun Liu; Zamani, Arezoo; Ekramoddoullah, Abul K. M.
- Abstract
The protein content in the plant apoplast is believed to change dramatically as a result of host defense response upon infection with various pathogens. In this study, six novel thaumatin-like proteins (TLPs) were identified in western white pine ( Pinus monticola) needle apoplast by a proteomic strategy using two-dimensional protein electrophoresis followed by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. Sequent cDNA cloning found that ten P. monticola TLP genes ( PmTLP-L1 to -L6 and -S1 to -S4) were expressed in various tissues. Phylogenetic analysis demonstrated that these PmTLP genes belong to a large, complex, and highly diverse plant TLP family. Quantitative reverse transcription-polymerase chain reaction (RT-qPCR) using gene-specific primer pairs showed that each PmTLP gene exhibited a characteristic pattern of mRNA expression based on their unique organ distribution, seasonal regulation, and response to abiotic and biotic stresses. A time-course analysis at the early stages of infection by white pine blister rust pathogen Cronartium ribicola revealed that a coordinated upregulation of multiple PmTLP genes was involved in P. monticola major gene ( Cr2) resistance. The structural and expressional differentiations suggest that the PmTLP family may contribute to host defense as well as other mechanism.
- Subjects
THAUMATINS; PROTEINS; WHITE pine; POLYMERASE chain reaction; MESSENGER RNA
- Publication
Planta: An International Journal of Plant Biology, 2010, Vol 231, Issue 3, p637
- ISSN
0032-0935
- Publication type
Article
- DOI
10.1007/s00425-009-1068-2