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- Title
Genome sequencing and analysis of an Escherichia coli phage vB_EcoM-ep3 with a novel lysin, Lysep3.
- Authors
Lv, Meng; Wang, Shuang; Yan, Guangmou; Sun, Changjiang; Feng, Xin; Gu, Jingmin; Han, Wenyu; Lei, Liancheng
- Abstract
Bacteriophages represent one prospect for preventing and treating multi-drug-resistant Escherichia coli. In this study, we have isolated a novel E. coli-specific bacteriophage and characterised its biological properties. vB_EcoM-ep3 has a broad host range and was able to lyse 9 out of 15 clinical isolates of multi-drug-resistant pathogenic E. coli from chickens. The optimal multiplicity of infection for vB_EcoM-ep3 in host bacteria was 0.01. vB_EcoM-ep3 was thermostable at temperatures below 50 °C for up to 60 min. Electron microscopy demonstrated that vB_EcoM-ep3 belongs to Myoviridae. The vB_EcoM-ep3 genome contained 42,351 pairs of nucleotides with a GC content of 53.35 %. There were 52 predicted open reading frames that appeared to overlap and have a modular structure. Phylogenetic analysis indicates that the closest evolutionary relative to vB_EcoM-ep3 is the previously reported E. coli phage vB_EcoM_ECO1230-10. However, there was no homology between reported E. coli phage lysins and the vB_EcoM-ep3 lysin gene. Lysep3 was 58 % similar to the Pseudomonas phage PPpW-3 lysin despite showing no similarities at the gene sequence level. And Lysep3 has good lysis activity.
- Subjects
BACTERIAL genomes; BACTERIAL genetics; ESCHERICHIA coli; LYSINE; BACTERIOPHAGES; PATHOGENIC bacteria; TEMPERATURE; ELECTRON microscopy
- Publication
Virus Genes, 2015, Vol 50, Issue 3, p487
- ISSN
0920-8569
- Publication type
Article
- DOI
10.1007/s11262-015-1195-8