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- Title
Biochemical changes and their relationship with morphological and functional findings in pig heart subjected to lasting volume overload: a possible role of acylphosphatase in the regulation of sarcoplasmic reticulum calcium pump.
- Authors
Nediani, C.; Formigli, L.; Perna, A. M.; Pacini, A.; Ponziani, V.; Modesti, P. A.; Ibba-Manneschi, L.; Zecchi-Orlandini, S.; Fiorillo, C.; Cecchi, C.; Liguori, P.; Fratini, G.; Vanni, S.; Nassi, P.
- Abstract
We evaluated the changes in sarcoplasmic reticulum (SR) function and the parallel hemodynamic and morphological modifications in a heart subjected to volume overload. We also determined the levels of acylphosphatase, a cytosolic enzyme, that could play a regulatory effect on SR Ca2+ pump by hydrolyzing the phosphorylated intermediate of this transport system. For this, swine hearts were subjected to volume overload by aorta-cava shunt for 1, 2, or 3 months. Changes in heart contractility reflected modifications of SR function, whose reduction after 1 month of overload was followed by a gradual recovery. A decrease in SERCA2a protein and mRNA content was shown from 1 month and remained for the following 2 months. Phospholamban content and its phosphorylation status were not modified. Acylphosphatase was unchanged at 1 month, but at 2 months this enzyme exhibited an increased activity, protein and mRNA expression. Morphological alterations consisting of the cytoskeletal architectures, intermyofibrillar oedema, swollen mithochondria and abnormality of the membrane system (T-tubule and SR cisternae) were particularly evident after 1 month but almost disappeared after 3 months. These results suggest that our overloaded hearts underwent a substantial recovery of their structural and biochemical properties at 3 months after surgery. A possible involvement of acylphosphatase in the modification of SR function is discussed.
- Subjects
SARCOPLASMIC reticulum; MUSCLE cells; ENZYMES; MESSENGER RNA; BLOOD circulation; CHEMICAL reactions
- Publication
Basic Research in Cardiology, 2002, Vol 97, Issue 6, p469
- ISSN
0300-8428
- Publication type
Article
- DOI
10.1007/s00395-002-0367-6