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- Title
Microbial Production of Retinyl Palmitate and Its Application as a Cosmeceutical.
- Authors
Choi, Bo Hyun; Hwang, Hee Jin; Lee, Ji Eun; Oh, Soon Hwan; Hwang, Jae Sung; Lee, Bun Yeoul; Lee, Pyung Cheon
- Abstract
Chemically synthesized retinyl palmitate has been widely used in the cosmetic and biotechnology industry. In this study, we aimed to demonstrate the microbial production of retinyl palmitate and the benefits of microbial retinyl palmitate in skin physiology. A heterologous retinyl palmitate biosynthesis pathway was reconstructed in metabolically engineered Escherichia coli using synthetic expression modules from Pantoea agglomerans, Salinibacter ruber, and Homo sapiens. High production of retinyl palmitate (69.96 ± 2.64 mg/L) was obtained using a fed-batch fermentation process. Moreover, application of purified microbial retinyl palmitate to human foreskin HS68 fibroblasts led to increased cellular retinoic acid-binding protein 2 (CRABP2) mRNA level [1.7-fold (p = 0.001) at 100 μg/mL], acceleration of cell proliferation, and enhancement of procollagen synthesis [111% (p < 0.05) at 100 μg/mL], strongly indicating an anti-ageing-related effect of this substance. These results would pave the way for large-scale production of retinyl palmitate in microbial systems and represent the first evidence for the application of microbial retinyl palmitate as a cosmeceutical.
- Subjects
SKIN physiology; MESSENGER RNA; BIOTECHNOLOGY industries; COSMETICS industry; ESCHERICHIA coli; HYPERTROPHIC scars
- Publication
Antioxidants, 2020, Vol 9, Issue 11, p1130
- ISSN
2076-3921
- Publication type
Article
- DOI
10.3390/antiox9111130