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- Title
Inhibition of Orientia tsutsugamushi infection by a truncated recombinant 56-kDa outer membrane protein.
- Authors
Park, S.; Hwang, K. J.; Chu, H.; Park, S. H.; Shim, S. K.; Choi, Y. S.; Kim, J. S.; Park, M. Y.
- Abstract
Aims: The objective of this study was to evaluate recombinant 56-kDa outer membrane protein as a potential inhibitor to infection from Orientia tsutsugamushi. Methods and Results: The 56-kDa protein was cloned and expressed in an Escherichia coli system, and the degree of target cell attachment to immobilized 56-kDa protein was measured in a cell adhesion assay. The results showed that the 56-kDa protein has an ability to attach HeLa cells. Furthermore, treatment of target cells with a truncated 56-kDa 1–418 (amino acid residues) protein inhibited target cell infection by O. tsutsugamushi, demonstrated with an indirect immunofluorescence antibody assay. Conclusions: The truncated 56-kDa protein (a.a. 1–418) plays an important role in O. tsutsugamushi infection, and the 56-kDa protein could be useful and effective in the inhibition of O. tsutsugamushi attachment and infection. Significance and Impact of the Study: The attachment of the 56-kDa protein to target cells was directly determined by in vitro adherence test, and the invasion of target cells by O. tsutsugamushi was inhibited by treating the target cells with a truncated 56-kDa protein.
- Subjects
TSUTSUGAMUSHI disease; MEMBRANE proteins; IMMUNOFLUORESCENCE; CELL communication; CANCER cells
- Publication
Letters in Applied Microbiology, 2010, Vol 50, Issue 5, p445
- ISSN
0266-8254
- Publication type
Article
- DOI
10.1111/j.1472-765X.2010.02814.x