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- Title
粤紫萁离体再生技术研究.
- Authors
陈 朋; 杨蕾蕾; 彭 杨; 张苏州; 徐桂红; 杨建芬; 张寿洲
- Abstract
Osmunda milda is a recently formed natural hybrid species with a riarrow distribution area. The number of the population is very small, and its sexual reproduction in extremely low. Using spores as explants, we investigated the effects of basic media (MS, 3/4 MS, 1/2 MS and 1/4 MS) and different plant growth regulators (PCRs) on spores germination, prothallium proliferation and sporophyte formation. The results were as follows: Extremely few fertile spores of 0. mildea could germinate and develop into prothallus in effect of plant growth regulators; The optimal PCR combination of prothallus growth and proliferation were 1/2MS +1.0 mg L-16-BA+0.05 mg L-1 NAA and 1/2MS + 1.0 mg L-16-BA+0,5 mg L-1 IBA; In the 1/4MS with no PGR medium, prothallus were well developed with the proliferation, and the young sporophyte seedling formatted, the formation of scale was 83.49%: The best rooting PCR concentration for young sporophyte was MS+0.5 mg L-1IBA; In loess : river sand : humus 1: 1:3 cultivation matrix was the best, the survival rate was more than 98%, and the seedlings thrived. This study is great significance to the artificial breeding and conservation of O. mildea, and also provides reference to the artificial conservation of other endangered fern species.
- Subjects
PLANT regulators; ENDANGERED species; SPECIES distribution; PLANT reproduction; SPORES; FERNS; ENDANGERED plants
- Publication
Guihaia, 2021, Vol 41, Issue 2, p301
- ISSN
1000-3142
- Publication type
Article
- DOI
10.11931/guihaiia.gxzw201908045