We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Comparison of Six Commercial Diagnostic Tests for the Detection of Dengue Virus Non-Structural-1 Antigen and IgM/IgG Antibodies.
- Authors
Hyeyoung Lee; Ji Hyeong Ryu; Hye-Sun Park; Ki Hyun Park; Hyunjin Bae; Sojeong Yun; Ae-Ran Choi; Sung-Yeon Cho; Chulmin Park; Dong-Gun Lee; Jihyang Lim; Jehoon Lee; Seungok Lee; Soyoung Shin; Haeil Park; Eun-Jee Oh
- Abstract
ELISAs and rapid diagnostic tests (RDTs) are widely used for diagnosing dengue virus (DENV) infection. Using 138 single blood samples, we compared the ability to detect nonstructural (NS)-1 antigen and anti-DENV IgM/IgG antibodies among (1) DENV Detect NS1 ELISA, DENV Detect IgM capture ELISA and DENV Detect IgG ELISA (InBios International, Inc.); (2) Anti-Dengue virus IgM Human ELISA and Anti-Dengue virus IgG Human ELISA (Abcam); (3) Dengue virus NS1 ELISA, Anti-Dengue virus ELISA (IgM) and Anti-Dengue virus ELISA (IgG) (Euroimmun); (4) Asan Easy Test Dengue NS1 Ag 100 and Asan Easy Test Dengue IgG/IgM (Asan Pharm); (5) SD BIOLINE Dengue Duo (Standard Diagnostics); and (6) Ichroma Dengue NS1 and Ichroma Dengue IgG/IgM (Boditech Med). For NS1 antigen detection, InBios and Euroimmun showed higher sensitivities (100%) than the RDTs (42.9-64.3%). All tests demonstrated variable sensitivities for IgM (38.1-90.5%) and IgG (65.7-100.0%). InBios and Boditech Med demonstrated higher sensitivity (95.6% and 88.2%, respectively) than the other tests for combined NS1 antigen and IgM antibody. Five NS1 antigen tests had good agreement (92.8-98.6%) without showing positivity for chikungunya. However, all IgG tests demonstrated potential false-positivity with variable ranges. Clinical laboratories should note performance variations across tests and potential cross-reactivity.
- Subjects
IMMUNOGLOBULIN M; DENGUE viruses; DIAGNOSIS methods; IMMUNOGLOBULINS; ANTIGENS; DENGUE
- Publication
Annals of Laboratory Medicine, 2019, Vol 39, Issue 6, p566
- ISSN
2234-3806
- Publication type
Article
- DOI
10.3343/alm.2019.39.6.566