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- Title
2,3-Dimethoxycinnamic Acid from a Marine Actinomycete, a Promising Quorum Sensing Inhibitor in Chromobacterium violaceum.
- Authors
Li, Yanqun; Ding, Wenping; Yin, Jiajia; Li, Xingyu; Tian, Xinpeng; Xiao, Zhihui; Wang, Fazuo; Yin, Hao
- Abstract
An ethyl acetate extract of a marine actinomycete strain, Nocardiopsis mentallicus SCSIO 53858, isolated from a deep-sea sediment sample in the South China Sea, exhibited anti-quorum-sensing (QS) activity against Chromobacterium violaceum CV026. Guided by the anti-QS activity, a novel active compound was isolated and purified from the extract and was identified as 2,3-dimethoxycinnamic acid (2,3-DCA) through spectral data analysis. At a concentration of 150 μg/mL, 2,3-DCA exhibited robust inhibitory effects on three QS-regulated traits of C. violaceum CV026: violacein production, swarming motility, and biofilm formation, with inhibition rates of 73.9%, 65.9%, and 37.8%, respectively. The quantitative reverse transcription polymerase chain reaction results indicated that 2,3-DCA can disrupt the QS system in C. violaceum CV026 by effectively suppressing the expression of QS-related genes, including cviR, vioA, vioB, and vioE. Molecular docking analysis revealed that 2,3-DCA hinders the QS system by competitively binding to the same binding pocket on the CviR receptor as the natural signal molecule N-hexanoyl-L-homoserine lactone. Collectively, these findings suggest that 2,3-DCA exhibits promising potential as an inhibitor of QS systems, providing a potential solution to the emerging problem of bacterial resistance.
- Subjects
QUORUM sensing; CHROMOBACTERIUM violaceum; REVERSE transcriptase polymerase chain reaction; CANDIDA albicans; STREPTOMYCES; DRUG resistance in bacteria; MOLECULAR docking
- Publication
Marine Drugs, 2024, Vol 22, Issue 4, p177
- ISSN
1660-3397
- Publication type
Article
- DOI
10.3390/md22040177