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- Title
Backfilling-Free Strategy for Biopatterning on Intrinsically Dual-Functionalized Poly[2-Aminoethyl Methacrylate- co-Oligo(Ethylene Glycol) Methacrylate] Films.
- Authors
Lee, Bong Soo; Lee, Juno; Han, Gyeongyeop; Ha, EunRae; Choi, Insung S.; Lee, Jungkyu K.
- Abstract
We demonstrated protein and cellular patterning with a soft lithography technique using poly[2-aminoethyl methacrylate- co-oligo(ethylene glycol) methacrylate] films on gold surfaces without employing a backfilling process. The backfilling process plays an important role in successfully generating biopatterns; however, it has potential disadvantages in several interesting research and technical applications. To overcome the issue, a copolymer system having highly reactive functional groups and bioinert properties was introduced through a surface-initiated controlled radical polymerization with 2-aminoethyl methacrylate hydrochloride (AMA) and oligo(ethylene glycol) methacrylate (OEGMA). The prepared poly(AMA- co-OEGMA) film was fully characterized, and among the films having different thicknesses, the 35 nm-thick biotinylated, poly(AMA- co-OEGMA) film exhibited an optimum performance, such as the lowest nonspecific adsorption and the highest specific binding capability toward proteins.
- Subjects
ETHYLENE crystals; ETHYLENE; COPOLYMERS; ETHYLENE glycols; ETHYLENE glycol
- Publication
Chemistry - An Asian Journal, 2016, Vol 11, Issue 14, p2057
- ISSN
1861-4728
- Publication type
Article
- DOI
10.1002/asia.201600585