We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Three-Dimensional Reconstruction of Oral Tongue Squamous Cell Carcinoma at Invasion Front.
- Authors
Tomoo Kudo; Yoshihito Shimazu; Hisao Yagishita; Toshiyuki Izumo; Yuuichi Soeno; Kaori Sato; Yuji Taya; Takaaki Aoba
- Abstract
We conducted three-dimensional (3D) reconstruction of oral tongue squamous cell carcinoma (OTSCC) using serial histological sections to visualize the architecture of invasive tumors. Fourteen OTSCC cases were collected from archival paraffin-embedded specimens. Based on a pathodiagnostic survey of whole cancer lesions, a core tissue specimen (3mm in diameter) was dissected out from the deep invasion front using a paraffin tissue microarray. Serial sections (4 Μm thick) were double immunostained with pan-cytokeratin and Ki67 antibodies and digitized images were acquired using virtual microscopy. For 3D reconstruction, image registration and RGB color segmentation were automated using Image J software to avoid operator-dependent subjective errors. Based on the 3D tumor architecture, we classified the mode of invasion into four types: pushing and bulky architecture; trabecular architecture; diffuse spreading; and special forms. Direct visualization and quantitative assessment of the parenchymal-stromal border provide a new dimension in our understanding of OTSCC architecture. These 3D morphometric analyses also ascertained that cell invasion (individually and collectively) occurs at the deep invasive front of the OTSCC. These results demonstrate the advantages of histology-based 3D reconstruction for evaluating tumor architecture and its potential for a wide range of applications.
- Subjects
SQUAMOUS cell carcinoma; PLASTIC surgery; TONGUE surgery; HISTOLOGY; MICROARRAY technology; MORPHOMETRICS
- Publication
International Journal of Dentistry, 2013, p1
- ISSN
1687-8728
- Publication type
Article
- DOI
10.1155/2013/482765