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- Title
Purification of innate immunostimulant from green tea using a silkworm muscle contraction assay.
- Authors
Dhital, Saphala; Hamamoto, Hiroshi; Urai, Makoto; Ishii, Kenichi; Sekimizu, Kazuhisa
- Abstract
A polysaccharide was purified from a hot water extract of green tea leaves by measuring the immunostimulatory activity in silkworm larvae. Nuclear magnetic resonance and chemical analysis of acid hydrolysates revealed that the purified substance possessed a backbone containing polygalacturonic acids with methyl ester residues. Treatment with β-glucanase attenuated the muscle contraction activity of the purified sample, suggesting that the β-glucan structure, probably as a branched form, was required for its activity. The purified fraction stimulated the production of interleukin-6 by mouse peritoneal macrophages. These results suggest that measuring immunostimulation in silkworm larvae is useful for evaluating innate immunostimulants from various sources.
- Subjects
POLYSACCHARIDES; PECTINS; GREEN tea; NATURAL immunity; MACROPHAGES; INTERLEUKIN-6; ANALYTICAL chemistry; MUSCLE contraction; PREVENTION; THERAPEUTICS
- Publication
Drug Discoveries & Therapeutics, 2011, Vol 5, Issue 1, p18
- ISSN
1881-7831
- Publication type
Article
- DOI
10.5582/ddt.v5.1.18