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- Title
Effects of neuropeptide Y on the cerebral vascular smooth muscle cell proliferation and α-actin expression after subarachnoid hemorrhage.
- Authors
XUE Jun-gang; SONG Jin-ning; AN Ji-yang; WANG Jun-feng; LI Dan-dong; ZHANG Ming; SUN Peng; LI Yu
- Abstract
Objective To study the role of neuropeptide Y (NPY) in vasospasm after subarachnoid hemorrhage (SAH) in vitro cultured rat cerebral vascular smooth muscle cells (VSMCs) by observing the proliferation and contraction protein expression of VSMCs. Methods Male or female SD rats weighing 150 g were killed by blood loss. After infusion with saline, the circle of Willis artery and its branches were harvested and primary VSMCs were cultured. Then in vitro model of SAH was established by exposure to oxyhemoglobin of 10-6 mol/L. Then the cells were treated with NPY or BIB03304 (a commonly used blocker of NPY receptor), or NPY + BIB03304. After culturing 1,3,5 and 7 days, immunofluorescence was used to measure the expression of α-actin. IPP software was used to measure the fluorescence intenty and SPSS16.0 statistical software was used for data analysis. Results VSMCs were successfully cutured whli digettion method. The fifth generation of visible cells showed a typical "valley-peak"-like growth. In NPY and NPY + SAH groups, 3-day cell intervention significantly increased the fluorescence intensity of α-actin (P<0. 05), while BIBO 3304 treatment reduced the expression of α-actin, the intensity of which was as weak as that of the blank group (P>0.05). Conclusion NPY increases α-actin expression in VSMCs while BIBO 3304 blocks the up-regulation of α-acin by NPY. NPY plays an important role in delayed cerebral vasospasm after SAH.
- Subjects
NEUROPEPTIDE Y; VASCULAR smooth muscle; MUSCLE cells; CELL proliferation; ACTIN; SUBARACHNOID hemorrhage; CEREBRAL vasospasm
- Publication
Journal of Xi'an Jiaotong University (Medical Sciences), 2013, Vol 34, Issue 6, p744
- ISSN
1671-8259
- Publication type
Article
- DOI
10.7652/jdyxb201306009