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- Title
Improvement of Wild Bacterial Strain NIAB-SM-3 for Better Lysine Production Using N-methyl-N'-nitro-N-nitrosoguanidine (NTG).
- Authors
Nadeem, Shahid; Muhammad Tauseef Sultan; Masood Sadiq Butt; Saima Hafeez Khan; Hafiz Muhammad Muzzamil; Niaz, Bushra
- Abstract
Classical chemical mutagenesis is one of the important tools in improving the bacterial strains for the production of desired metabolites. The N-methyl-N'-nitro-N-nitrosoguanidine (NTG) is one of the commonly employed mutagen for the improvement of bacterial strain for better amino acid production. In the present research, wild strain NIAB-SM-3 was isolated from molasses dumped soil. It produced 3.0g/L of lysine along with glutamic acid and alanine when grown in molasses based medium. This wild strain was mutated with the help of NTG. Minimum inhibitory concentrations (MIC) for NTG and lysine analogue, S-(β-aminoethyl)-L-cysteine (AEC), were found to be 50µg/ml and 625µg/ml, respectively. On the basis of AEC resistance 43 out of total 57 mutants were selected and tested on different media for their abilities to produce lysine in fermentation broth. Among the molasses media, NIAB-NT-43 gave maximum lysine production i.e. 3.8g/L in M-I medium. On the basis of their lysine production in molasses media, 16 mutants were selected and cultured in glucose medium, L-6. Almost all of them produced lysine but maximum production of 5.0g/L was observed by NIAB-NT-15. In the nutshell, two-fold increase in lysine was observed as a result of chemical mutagenesis through the use of NTG. Furthermore, repeated classical chemical mutagenesis and tools of genetic engineering can be employed in the future to enhance lysine production.
- Subjects
ESCHERICHIA coli; NITROSOGUANIDINE; AMINO acids; LYSINE; MOLASSES; MUTAGENESIS
- Publication
Pakistan Journal of Zoology, 2013, Vol 45, Issue 6, p1489
- ISSN
0030-9923
- Publication type
Article