We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Association of Mouse Sorting Nexin 1 with Early Endosomes1.
- Authors
Nakamura, Norihiro; Sun-Wada, Ge-Hong; Yamamoto, Akitsugu; Wada, Yoh; Futai, Masamitsu
- Abstract
Sorting nexin 1 (SNX1) is a protein that binds to the cytoplasmic domain of plasma membrane receptors. We found that mouse sorting nexin 1 (SNX1) (521 amino acid residues) could partially rescue a yeast vam3 mutant defective in docking/fusion of vacuolar membranes. In mnmnialimi cells, SNX1 is peripherally associated with membrane structures and localized immunochemically with EEA1, a marker protein of early endosomes. These results suggest that SNXl regulates endocytic trafficking of plasma membrane proteins in early endosomes. Gel filtration of cell lysates and the purified recombinant protein, together with two-hybrid analysis, indicated that SNXl self-assembles into a complex of ∼300 kDa
- Subjects
SORTING nexins; AMINO acids; ENDOSOMES; MEMBRANE proteins; RECOMBINANT proteins; CELL membranes
- Publication
Journal of Biochemistry, 2001, Vol 130, Issue 6, p765
- ISSN
0021-924X
- Publication type
Article
- DOI
10.1093/oxfordjournals.jbchem.a003047