We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Deciphering the gene expression profile of peroxisome proliferator-activated receptor signaling pathway in the left atria of patients with mitral regurgitation.
- Authors
Mien-Cheng Chen; Jen-Ping Chang; Yu-Sheng Lin; Kuo-Li Pan; Wan-Chun Ho; Wen-Hao Liu; Tzu-Hao Chang; Yao-Kuang Huang; Chih-Yuan Fang; Chien-Jen Chen; Chen, Mien-Cheng; Chang, Jen-Ping; Lin, Yu-Sheng; Pan, Kuo-Li; Ho, Wan-Chun; Liu, Wen-Hao; Chang, Tzu-Hao; Huang, Yao-Kuang; Fang, Chih-Yuan; Chen, Chien-Jen
- Abstract
<bold>Background: </bold>Differentially expressed genes in the left atria of mitral regurgitation (MR) pigs have been linked to peroxisome proliferator-activated receptor (PPAR) signaling pathway in the KEGG pathway. However, specific genes of the PPAR signaling pathway in the left atria of MR patients have never been explored.<bold>Methods: </bold>This study enrolled 15 MR patients with heart failure, 7 patients with aortic valve disease and heart failure, and 6 normal controls. We used PCR assay (84 genes) for PPAR pathway and quantitative RT-PCR to study specific genes of the PPAR pathway in the left atria.<bold>Results: </bold>Gene expression profiling analysis through PCR assay identified 23 genes to be differentially expressed in the left atria of MR patients compared to normal controls. The expressions of APOA1, ACADM, FABP3, ETFDH, ECH1, CPT1B, CPT2, SLC27A6, ACAA2, SMARCD3, SORBS1, EHHADH, SLC27A1, PPARGC1B, PPARA and CPT1A were significantly up-regulated, whereas the expression of PLTP was significantly down-regulated in the MR patients compared to normal controls. The expressions of HMGCS2, ACADM, FABP3, MLYCD, ECH1, ACAA2, EHHADH, CPT1A and PLTP were significantly up-regulated in the MR patients compared to patients with aortic valve disease. Notably, only ACADM, FABP3, ECH1, ACAA2, EHHADH, CPT1A and PLTP of the PPAR pathway were significantly differentially expressed in the MR patients compared to patients with aortic valve disease and normal controls.<bold>Conclusions: </bold>Differentially expressed genes of the PPAR pathway have been identified in the left atria of MR patients compared with patients with aortic valve disease and normal controls.
- Subjects
GENE expression; PEROXISOME proliferator-activated receptors; MITRAL valve insufficiency; AORTIC valve diseases; GENE expression profiling; CELL metabolism; RNA metabolism; CELLULAR signal transduction; FATTY acids; HEART atrium; HEART failure; LIPIDS; OXIDATION-reduction reaction; POLYMERASE chain reaction; RESEARCH evaluation; RNA; STAINS &; staining (Microscopy); CASE-control method
- Publication
Journal of Translational Medicine, 2016, Vol 14, p1
- ISSN
1479-5876
- Publication type
journal article
- DOI
10.1186/s12967-016-0871-3