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- Title
Protective immunity of SpaA-antigen producing Lactococcus lactis against Erysipelothrix rhusiopathiae infection.
- Authors
Cheun, H. I.; Kawamoto, K.; Hiramatsu, M.; Tamaoki, H.; Shirahata, T.; Igimi, S.; Makino, S.-I.
- Abstract
h.i. cheun, k. kawamoto, m. hiramatsu, h. tamaoki, t. shirahata, s. igimi and s.-i. makino. 2004. To develop an economical, safe and simple vaccination system against swine erysipelas using SpaA-antigen producing Lactococcus lactis. The spaA gene of Erysipelothrix rhusiopathiae was inserted into a shuttle plasmid pSECE1 to construct pSECE1·3. The SpaA produced in L. lactis maintained a stable antigenicity without degrading in growth. After mice were inoculated intranasally and orally with pSECE1·3-carrying L. lactis cells, IgG and IgA specific to SpaA were detected, and all the mice survived a challenge with 100 LD50 of E. rhusiopathiae Tama-96 in the inner thigh. SpaA-producing L. lactis appears useful as an effective subunit vaccine against swine erysipelas. In this vaccination system, purification of the antigen and injection are unnecessary, leading to a reduced production cost, reduced labour and less stress to the animals. This vaccination system of the lactic acid bacteria should be a safe and suitable vehicle for a polyvalent vaccine.
- Subjects
VACCINATION; ERYSIPELOTHRIX rhusiopathiae; LACTOCOCCUS lactis; ANTIGENS; CELLS; BACTERIA; VACCINES
- Publication
Journal of Applied Microbiology, 2004, Vol 96, Issue 6, p1347
- ISSN
1364-5072
- Publication type
Article
- DOI
10.1111/j.1365-2672.2004.02283.x