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- Title
Estimating the relapse stage in chronic myeloid leukaemia patients after allogeneic stem cell transplantation by the amount of BCR-ABL fusion transcripts detected using a new real-time polymerase chain reaction method.
- Authors
Elmaagacli, Ahmet H.; Freist, Annette; Hahn, Meinhard; Opalka, Bertram; Seeber, Siegfried; Schaefer, Ulrich W.; Beelen, D. W.
- Abstract
We have used a new single-step real-time reverse transcription polymerase chain reaction (RT-PCR) method to quantify BCR-ABL transcripts, thereby estimating the relapse stage in chronic myeloid leukaemia patients after allogeneic transplants. In 402 samples from 172 patients, BCR-ABL expression was determined and normalized, using the GAPDH housekeeping gene product as an endogenous reference. In our real-time RT-PCR assay, serial dilutions of RNA of the K562 cell line remained positive down to 7·5 pg. The median normalized BCR-ABL amount differed significantly (P < 0·001) between the various disease stages and was 0·06% (range 0·001–1·55%), 3·2% (range 1·4–5·6%) and 21·5% (range 6·8 -827%) in 17 patients with a molecular relapse, in eight patients with a cytogenetic relapse and in 10 patients with a haematological relapse respectively.
- Subjects
POLYMERASE chain reaction; MYELOID leukemia
- Publication
British Journal of Haematology, 2001, Vol 113, Issue 4, p1072
- ISSN
0007-1048
- Publication type
Article
- DOI
10.1046/j.1365-2141.2001.02858.x