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- Title
Mucosal Concentrations of Proinflammatory Cytokines and Chemokines at Gastric Cardia: Implication ofHelicobacter pyloriInfection and Gastroesophageal Reflux.
- Authors
Isomoto, Hajime; Nishi, Yoshito; Wang, Aiping; Takeshima, Fuminao; Omagari, Katsuhisa; Mizuta, Yohei; Shikuwa, Saburo; Murata, Ikuo; Kohno, Shigeru
- Abstract
OBJECTIVES: The pathogenesis of carditis remains unclear, although gastroesophageal reflux disease (GERD) andHelicobacter pyloriinfection have been proposed. Little is known about the profile of proinflammatory cytokines and chemokines in the pathogenesis of carditis.METHODS: We studied 28 patients with GERD and 40 controls. Two biopsy specimens were taken endoscopically from the cardiac mucosa within 5 mm from the squamocolumnar junction; one was snap frozen for measurement of mucosal levels of interleukin 1β (IL-1β), tumor necrosis factor-α, IL-6, IL-8, monocyte chemoattractant protein 1 (MCP-1), regulated on activation normal T-cell expressed and presumably secreted (RANTES) by enzyme-linked immunosorbent assays, while another was processed for histopathology.H. pyloriinfection was assessed by serology, rapid urease test, and histology with Giemsa staining. Samples were taken from the cardia of 18H. pylori-positive patients, before and after eradication treatment.RESULTS: Carditis was significantly associated withH. pyloriinfection, but not GERD. IL-8, MCP-1, and RANTES levels were significantly higher in cardiac mucosa of patients with carditis than in those without it and in patients with than withoutH. pyloriinfection. IL-8 concentrations were significantly associated with the degree of neutrophil infiltration within the cardiac mucosa and decreased after cure of the infection. Mucosal MCP-1 and RANTES levels correlated positively with the grades of mononuclear cell infiltration and IL-1β concentrations.CONCLUSION: Our results indicate that chemokines produced locally in the cardiac mucosa may be involved in the development ofH. pylori-associated carditis.
- Subjects
CYTOKINES; CHEMOKINES; GASTROESOPHAGEAL reflux; HELICOBACTER pylori; GASTROENTEROLOGY; MEDICAL research
- Publication
American Journal of Gastroenterology (Springer Nature), 2004, Vol 99, Issue 6, p1063
- ISSN
0002-9270
- Publication type
Article
- DOI
10.1111/j.1572-0241.2004.30847.x