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- Title
Multiple metabolic pathways for metabolism of l-tryptophan in Fusarium graminearum.
- Authors
Luo, Kun; DesRoches, Caro-Lyne; Johnston, Anne; Harris, Linda J.; Zhao, Hui-Yan; Ouellet, Thérèse
- Abstract
Fusarium graminearum is a plant pathogen that can cause the devastating cereal grain disease fusarium head blight in temperate regions of the world. Previous studies have shown that F. graminearum can synthetize indole-3-acetic acid (auxin) using l-tryptophan (L-TRP)-dependent pathways. In the present study, we have taken a broader approach to examine the metabolism of L-TRP in F. graminearum liquid culture. Our results showed that F. graminearum was able to transiently produce the indole tryptophol when supplied with L-TRP. Comparative gene expression profiling between L-TRP-treated and control cultures showed that L-TRP treatment induced the upregulation of a series of genes with predicted function in the metabolism of L-TRP via anthranilic acid and catechol towards the tricarboxylic acid cycle. It is proposed that this metabolic activity provides extra energy for 15-acetyldeoxynivalenol production, as observed in our experiments. This is the first report of the use of L-TRP to increase energy resources in a Fusarium species.
- Subjects
TRYPTOPHAN; METABOLISM; FUSARIUM; PHYTOPATHOGENIC microorganisms; GENE expression profiling; MOLECULAR genetics
- Publication
Canadian Journal of Microbiology, 2017, Vol 63, Issue 11, p921
- ISSN
0008-4166
- Publication type
Article
- DOI
10.1139/cjm-2017-0383