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- Title
Response surface methodology for the optimization of keratinase production in culture medium containing feathers produced by Kocuria rosea.
- Authors
Bernal, C.; Diaz, I.; Coello, N.
- Abstract
A 43-fold increase in keratinase production by Kocuria rosea was achieved in batch fermentation using response surface methodology. Factorial designs were used to select the components of a culture medium that showed a significant effect on keratinase production. An orthogonal–central composite experimental design was performed, with only two (feathers and magnesium) from nine initial compounds being further analyzed by response surface methodology. An optimum keratinase production of 14 886.9 U/mg was obtained with the following medium composition (per litre): NH4Cl, 0.3 g; NaCl, 0.3 g; K2HPO4, 3.2 g; KH2PO4, 4.0 g; MgSO4·6H2O, 0.5 g; yeast extract, 0.1 g; and finely milled feathers, 30 g. The medium was shaken at 400 r/min with an incubation period of 14 h at 40 °C.
- Subjects
KERATIN; ENZYMES; CATALYSTS; PROTEINS; HYDROLYSIS
- Publication
Canadian Journal of Microbiology, 2006, Vol 52, Issue 5, p445
- ISSN
0008-4166
- Publication type
Article
- DOI
10.1139/W05-139