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- Title
Enrichment of Ammonia-Oxidizing Bacteria and Microbial Diversity Analysis by High-Throughput Sequencing.
- Authors
RENDA YAO; HONG YANG; MENGYU YU; YI LIU; HUAN SHI
- Abstract
A fully automatic miniature fermenter was operated to enrich ammoniaoxidizing bacteria (AOB) using the activated sludge obtained from the A2/O process in a wastewater treatment plant (WWTP) as the seed sludge. The results revealed that the AOB community can be enriched by a continuous ammonia feeding mode for intermittent operation, resulting in the appropriate regulation of free ammonia (FA) and free nitrous acid (FNA) levels. The maximum ammonia oxidation rate was over 170 mg/ (L·h) and nitrite accumulation ratio over 80%. High-throughput sequencing showed that Nitrosomonas belonging to AOB, were highly enriched after 27 days, having increased from 0.24% to 53.03%.
- Subjects
AMMONIA-oxidizing bacteria; MICROBIAL diversity; ACTIVATED sludge process; NITROUS acid; NITROSOMONAS
- Publication
Journal of Residuals Science & Technology, 2017, Vol 14, Issue 1, p77
- ISSN
1544-8053
- Publication type
Article
- DOI
10.12783/issn.1544-8053/14/1/10