We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Synthesis and characterisation of gelatin–nano hydroxyapatite composite scaffolds for bone tissue engineering.
- Authors
Mobini, S.; Javadpour, J.; Hosseinalipour, M.; Ghazi-Khansari, M.; Khavandi, A.; Rezaie, H. R.
- Abstract
Gelatin–hydroxyapatite (HAp) nanocomposites have been prepared by particulate leaching technique using glutaraldehyde (GTA) as cross-linking agent for polymer. The porosity in the scaffolds was controlled using sodium chloride as porogen agent. Microstructural investigation by scanning electron microscopy (SEM), revealed the formation of a well interconnected porous scaffold with pore size in the range of 100–200 μm. X-ray diffraction and Fourier transform infrared spectroscopy were used to confirm the formation of crystalline HAp as well the presence of both constituents in the composite samples. The bioactivity of the samples was evaluated by conducting MTT (3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide) assay and cell adhesion tests. The results suggest that the use of GTA in excess of 025% can be detrimental to cell survival. Cell attachment on the nanocomposite scaffold was verified by microscopic analysis.
- Subjects
GELATIN; HYDROXYAPATITE; SCANNING electron microscopy; FOURIER transform infrared spectroscopy; CELL adhesion
- Publication
Advances in Applied Ceramics: Structural, Functional & Bioceramics, 2008, Vol 107, Issue 1, p4
- ISSN
1743-6753
- Publication type
Article
- DOI
10.1179/174367608X246817