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- Title
The Aqueous Extract of Rhizome of Gastrodia elata Protected Drosophila and PC12 Cells against Beta-Amyloid-Induced Neurotoxicity.
- Authors
Chun-Fai Ng; Chun-Hay Ko; Chi-Man Koon; Jia-Wen Xian; Ping-Chung Leung; Kwok-Pui Fung; Ho Yin Edwin Chan; Clara Bik-San Lau
- Abstract
This study aims to investigate the neuroprotective effect of the rhizome of Gastrodia elata (GE) aqueous extract on beta-amyloid(Aβ)-induced toxicity in vivo and in vitro. Transgenic Drosophila mutants with Aβ-induced neurodegeneration in panneuron and ommatidia were used to determine the efficacy of GE. The antiapoptotic and antioxidative mechanisms of GE were also studied in Aβ-treated pheochromocytoma (PC12) cells. In vivo studies demonstrated that GE (5 mg/g Drosophila media)-treated Drosophila possessed a longer lifespan, better locomotor function, and less-degenerated ommatidia when compared with the Aβ-expressing control (all P < 0.05). In vitro studies illustrated that GE increased the cell viability of Aβ-treated PC12 cells in dose-dependent manner, probably through attenuation of Aβ-induced oxidative and apoptotic stress. GE also significantly up regulated the enzymatic activities of catalase, superoxide dismutase, and glutathione peroxidase, leading to the decrease of reactive oxidation species production and apoptotic marker caspase-3 activity. In conclusion, our current data presented the first evidence that the aqueous extract of GE was capable of reducing the Aβ-induced neurodegeneration in Drosophila, possibly through inhibition of apoptosis and reduction of oxidative stress. GE aqueous extract could be developed as a promising herbal agent for neuroprotection and novel adjuvant therapies for Alzheimer's disease.
- Subjects
CHINA; PHYTOTHERAPY; SYNDROMES; ACADEMIC medical centers; ALZHEIMER'S disease; ANALYSIS of variance; ANIMAL experimentation; BIOPHYSICS; CHI-squared test; FLOW cytometry; RESEARCH methodology; NEUROTOXICOLOGY; RESEARCH funding; STATISTICS; DATA analysis; DATA analysis software; IN vitro studies; LOG-rank test; THERAPEUTICS
- Publication
Evidence-based Complementary & Alternative Medicine (eCAM), 2013, Vol 2013, p1
- ISSN
1741-427X
- Publication type
Article
- DOI
10.1155/2013/516741