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- Title
Evaluation of the Chemical Reporter Analog PNP-6AzGlcNAc as an O- GlcNAcase Substrate.
- Authors
Kim, Eun J.; Bond, Michelle R.; Nam, Ghilsoo; Hanover, John A.
- Abstract
6-Azido-6-deoxy- N-acetylglucosamine ( 6AzGlcNAc) was recently introduced as a new selective metabolic chemical reporter ( MCR) for labeling O- GlcNAc-modified proteins in cells. To investigate whether O- 6AzGlcNAc is readily cleaved by O- GlcNAcase ( OGA), the enzyme responsible for removing the O- GlcNAc modification, we synthesized PNP-6AzGlcNAc. This analog mimics O- GlcNAc and can be used in vitro to define the kinetic parameters for OGA thereby defining whether O- 6AzGlcNAc can be employed as an appropriate tool to monitor O- GlcNAc dynamics in cells. Both PNP-6AzGlcNAc and PNP-GlcNAc were efficiently hydrolyzed by OGA with similar kinetics suggesting that an azido-modification at the GlcNAc C6 position does not significantly interfere with the β-hexosaminidase activity of OGA.
- Subjects
AZIDO group; METABOLOMICS; ENZYMES; HYDROLYSIS; HEXOSAMINIDASE
- Publication
Bulletin of the Korean Chemical Society, 2017, Vol 38, Issue 2, p264
- ISSN
0253-2964
- Publication type
Article
- DOI
10.1002/bkcs.11076