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- Title
Beta-lactamase Enzymes of Clinical Pseudomonas aeruginosa Strains.
- Authors
Pasa, O.; Ozer, B.; Duran, N.; Inci, M.; Yula, E.
- Abstract
Objectives: In this study, the production of extended spectrum beta-lactamase (ESBL), metallo-betalacatamase (MBL) and AmpC beta-lactamase enzymes of Pseudomonas aeruginosa (P aeruginosa) strains which were isolated from clinical samples were investigated. AmpC gene was also detected by the polymerase chain reaction (PCR) analysis. Methods: A hundred strains of P aeruginosa were included in the study. The presence of ESBL was investigated with combined disk confirmation test, MBL was investigated with E-test method and AmpC beta-lactamase was investigated with disk induction test. In order to detect the production of AmpC betalactamase genotypically, the PCR method was used. Results: Only one strain was found to be MBL positive. Four per cent of strains were found to be ESBL positive. AmpC beta-lactamase production was positive in 73% of the strains with disk induction test. AmpC gene was detected in 96% of the studied strains with the PCR method. Conclusion: While ESBL and MBL rates in this study were significantly lower than those found in other studies, the rate of AmpC beta-lactamase was higher. Although AmpC gene was detected in some strains (23%), they were not found to produce AmpC beta-lactamase with disk induction test
- Subjects
BETA-lactamase inhibitors; PSEUDOMONAS aeruginosa; POLYMERASE chain reaction; BETA lactamases; ENZYMES
- Publication
West Indian Medical Journal, 2016, Vol 65, Issue 1, p40
- ISSN
0043-3144
- Publication type
Article
- DOI
10.7727/wimj.2014.362