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- Title
A triple suicide gene strategy that improves therapeutic effects and incorporates multimodality molecular imaging for monitoring gene functions.
- Authors
Xing, L.; Sun, X.; Deng, X.; Kotedia, K.; Zanzonico, P. B.; Ackerstaff, E.; Koutcher, J. A.; Ling, C. C.; Li, G. C.
- Abstract
Gene-directed enzyme prodrug therapy (GDEPT), or suicide gene therapy, has shown promise in clinical trials. In this preclinical study using stable cell lines and xenograft tumor models, we show that a triple-suicide-gene GDEPT approach produce enhanced therapeutic efficacy over previous methods. Importantly, all the three genes (thymidine kinase, cytosine deaminase and uracil phosphoribosyltransferase) function simultaneously as effectors for GDEPT and markers for multimodality molecular imaging (MMI), using positron emission tomography, magnetic resonance spectroscopy and optical (fluorescent and bioluminescent) techniques. It was demonstrated that MMI can evaluate the distribution and function/activity of the triple suicide gene. The concomitant expression of these genes significantly enhances prodrug cytotoxicity and radiosensitivity in vitro and in vivo.
- Subjects
GENE therapy; CANCER treatment; IMAGING of cancer; POSITRON emission tomography; CANCER radiotherapy; XENOGRAFTS; ANTIBODY-directed enzyme prodrug therapy; TREATMENT effectiveness; CLINICAL trials; NUCLEAR magnetic resonance spectroscopy
- Publication
Cancer Gene Therapy, 2013, Vol 20, Issue 6, p358
- ISSN
0929-1903
- Publication type
Article
- DOI
10.1038/cgt.2013.28