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- Title
Vagotomy Decreases the Neuronal Activities of Medulla Oblongata and Alleviates Neurogenic Inflammation of Airways Induced by Repeated Intra-Esophageal Instillation of HCl in Guinea Pigs.
- Authors
Zhe CHEN; Hui CHEN; Fagui CHEN; Dachuan GU; Lejia SUN; Weitao ZHANG; Linfeng FAN; Yong LIN; Rong DONG; Kefang LAI
- Abstract
Neuronal activity in the medulla oblongata and neurogenic inflammation of airways were investigated in a guinea pig model induced by repeated intra-esophageal instillation of hydrochloric acid (HCl) after vagotomy. Unilateral vagotomy was performed in the vagotomy group, while a sham-operation was performed in the sham group. Operation was not conducted in sham control group. Airway inflammation was observed with hematoxylin and eosin (HE) staining. C-fos protein was measured by immunohistochemistry (IHC) and Western blot (WB). Substance P was examined by IHC and enzyme-linked immuno sorbent assay (ELISA). Airway microvascular permeability was detected by evans blue dye (EBD) fluorescence. Inflammation of airway was observed in the trachea and bronchi after chronic HCl perfusion into the lower esophagus, and was alleviated after unilateral vagotomy. C-fos expression in the medulla oblongata was lower in the vagotomy group compared to the sham control and sham groups. Substance P-like immunoreactivity (SP-li), concentration and microvascular leakage in airway were lower in the vagotomy group than that in the other groups. Our results suggest that vagotomy improved neurogenic inflammation of airways and decreased neuronal activities, the afferent nerves and neurons in medulla oblongata may be involved in neurogenic inflammation of airways mediated by esophageal-bronchial reflex.
- Subjects
VAGOTOMY; MEDULLA oblongata; ASTHMA; HYDROCHLORIC acid; EOSIN; GUINEA pigs as laboratory animals
- Publication
Physiological Research, 2017, Vol 66, Issue 6, p1021
- ISSN
0862-8408
- Publication type
Article
- DOI
10.33549/physiolres.933574