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- Title
Phylogenetic Relationships ofXylella fastidiosaStrains Based on 16S-23S rDNA Sequences.
- Authors
Martinati, Juliana Camargo; x00E1;via Tereza Hansen#Pacheco, Fl&; de Miranda, Vitor Fernandes Oliveira; Siu Mui Tsai
- Abstract
In spite of the lack of resolution ofXylella fastidiosaphylogenetic relationships, parsimony analysis of the 16S-23S rDNA sequence from a wide range of hosts has been evaluated in this research. In order to establish an easier method for sequencing the spacer region completely, a new primer pair was designed. The sequences obtained revealed a higher level of variation than that found in 16S gene sequences, with similarity values ranging from 0.80 to 1.00. The cladogram constructed allowed the clustering of two major clades. From these results it has been possible to recognize the monophyletic grouping of some strains belonging to the same host, possibly representing only one infection process. However, for other hosts there is paraphyletic and polyphyletic grouping. This methodology followed from promising results regarding strain-host clustering. With the parsimony approach, hypothetical genealogical relationship amongXylellastrains may be inferred.
- Subjects
PHYLOGENY; RECOMBINANT DNA; NUCLEOTIDE sequence; MICROORGANISMS; GENETIC code
- Publication
Current Microbiology, 2005, Vol 50, Issue 4, p190
- ISSN
0343-8651
- Publication type
Article
- DOI
10.1007/s00284-004-4405-5