We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Small subunit ribosomal RNA and mitochondrial cytochrome c oxidase subunit 1 gene sequences of 21 strains of the parasitic scuticociliate Miamiensis avidus (Ciliophora, Scuticociliatia).
- Authors
Jung, Sung-Ju; Im, Eun-Young; Strüder-Kypke, Michaela; Kitamura, Shin-Ichi; Woo, Patrick
- Abstract
The scuticociliate Miamiensis avidus is a histophagous parasite that causes high mortality in cultured marine fishes. Small subunit ribosomal RNA (SSU rRNA) and mitochondrial cytochrome c oxidase subunit 1 ( cox1) genes were analyzed for 21 strains of M. avidus isolated from diseased olive flounder ( Paralichthys olivaceus), ridged-eye flounder ( Pleuronichthys cornutus), and spotted knifejaw ( Oplegnathus fasciatus) in Korea and Japan (collected in 2003-2007). Analysis of SSU rRNA gene sequences (1,759 bp) indicates they are very conserved with less than 0.17% (3 nucleotides) differences suggesting that SSU rRNA are useful to identify M. avidus; however, the cox1 gene (900 bp) has higher variations with intraspecific divergences up to 5.67% (51 nucleotides). A distance tree of cox1 gene sequences based on a neighbor-joining analysis can separate 21 strains into five cox1 types (two heterogeneous clusters and three individual branches). The cox1-type matches with serotype of strains but do not reflect geographical origins, host species, or pathogenicity.
- Subjects
SCUTICOCILIATIDA; FISH parasites; FISH mortality; CYTOCHROME oxidase; PARALICHTHYS
- Publication
Parasitology Research, 2011, Vol 108, Issue 5, p1153
- ISSN
0932-0113
- Publication type
Article
- DOI
10.1007/s00436-010-2157-7