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- Title
Overexpression of Triticum durum TdAnn12 gene confers stress tolerance through scavenging reactive oxygen species in transgenic tobacco.
- Authors
Ben Saad, Rania; Harbaoui, Marwa; Ben Romdhane, Walid; Zouari, Nabil; Giang, Khong N.; Ben Hsouna, Anis; Brini, Faical
- Abstract
Plant annexins are proteins with multiple functions and roles in plant development and responses to abiotic stresses. We report here the functional analysis of the TdAnn12 annexin protein isolated from Triticum durum Desf. We have previously shown that TdAnn12 expression is highly induced by different abiotic stresses. In the present study, to investigate the physiological and biochemical stress-induced responses, we overexpressed TdAnn12 in tobacco. We demonstrate that transgenic tobacco plants expressing TdAnn12 exhibited enhanced tolerance to salt, osmotic stress and H2O2 at the seedling stage. Under greenhouse conditions, these plants showed tolerance to drought and salt stresses. Moreover, scavenging reactive oxygen species (ROS), higher chlorophyll content, lower lipid peroxidation levels and increased antioxidant activities (peroxidase, catalase and superoxide dismutase) were observed. Finally, accumulation of TdAnn12 in tobacco positively affects the regulation of some stress-related genes (MnSOD , APX1 , CAT1 , P5CS , NHX1 , SOS1 and DREB1A). TdAnn12 interacts directly or indirectly with stress-related genes that could stimulate an adaptive potential to gain tolerance which is not present in non-transgenic (NT) plants. Our results clearly show that overexpression of TdAnn12 in transgenic tobacco improves stress tolerance through the removal of ROS. Abiotic stresses negatively affect the growth and productivity of crop plants around the world. We report here the functional characterisation of TdAnn12 in transgenic tobacco and its involvement in salt and drought stress tolerance through the removal of reactive oxygen species. These results show that TdAnn12 could be a useful candidate gene for engineering abiotic stress tolerance in cultivated plants.
- Subjects
DURUM wheat; REACTIVE oxygen species; CROPS; CULTIVATED plants; ABIOTIC stress; DROUGHT tolerance; OSMOTIC pressure; TOBACCO industry laws
- Publication
Functional Plant Biology, 2019, Vol 46, Issue 10, p885
- ISSN
1445-4408
- Publication type
Article
- DOI
10.1071/FP18316