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- Title
A heterologous production of the Trametes hirsuta laccase in the fungus Penicillium canescens.
- Authors
Abyanova, A. R.; Chulkin, A. M.; Vavilova, E. A.; Fedorova, T. V.; Loginov, D. S.; Koroleva, O. V.; Benevolensky, S. V.
- Abstract
A heterologous protein expression in the fungus Penicillium canescens is described for the first time. The fungal strains producing Trametes hirsuta 072 accase under control of a highly efficient promoter of the P. canescens gene bgaS has been constructed. These strains efficiently transcribe the T. hirsuta 072 laccase gene with a correct intron splicing. Activity of the secreted heterologous laccase in the culture liquid reaches 3 U/ml, accounting for 98% of the total laccase activity, which demonstrates a high efficiency of heterologous secretion. The synthesized P. canescens laccase has the same molecular weight as the enzyme produced by T. hirsuta 072.
- Subjects
TRAMETES (Polyporaceae); LACCASE; PENICILLIUM; BIOLOGICAL transport; MOLECULAR weights
- Publication
Applied Biochemistry & Microbiology, 2010, Vol 46, Issue 3, p313
- ISSN
0003-6838
- Publication type
Article
- DOI
10.1134/S0003683810030117