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- Title
Structures of the tRNA export factor in the nuclear and cytosolic states.
- Authors
Cook, Atlanta G.; Fukuhara, Noemi; Jinek, Martin; Conti, Elena
- Abstract
Transfer RNAs are among the most ubiquitous molecules in cells, central to decoding information from messenger RNAs on translating ribosomes. In eukaryotic cells, tRNAs are actively transported from their site of synthesis in the nucleus to their site of function in the cytosol. This is mediated by a dedicated nucleo-cytoplasmic transport factor of the karyopherin-β family (Xpot, also known as Los1 in Saccharomyces cerevisiae). Here we report the 3.2 Å resolution structure of Schizosaccharomyces pombe Xpot in complex with tRNA and RanGTP, and the 3.1 Å structure of unbound Xpot, revealing both nuclear and cytosolic snapshots of this transport factor. Xpot undergoes a large conformational change on binding cargo, wrapping around the tRNA and, in particular, binding to the tRNA 5′ and 3′ ends. The binding mode explains how Xpot can recognize all mature tRNAs in the cell and yet distinguish them from those that have not been properly processed, thus coupling tRNA export to quality control.
- Subjects
TRANSFER RNA; MESSENGER RNA; RIBOSOMES; EUKARYOTIC cells; CELL nuclei; SACCHAROMYCES cerevisiae; SCHIZOSACCHAROMYCES pombe; ORGANELLES; SACCHAROMYCES
- Publication
Nature, 2009, Vol 461, Issue 7260, p60
- ISSN
0028-0836
- Publication type
Article
- DOI
10.1038/nature08394