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- Title
Detection of some β-lactamase Genes in Klebsiella pneumoniae Isolated from some Baghdad Hospitals, Iraq.
- Authors
Hussein, Ali Y.; Abdulsattar, Ban O.; Al-Saryi, Nadal A.; Edrees, Wadhah H.
- Abstract
Background: Multi-Drug-ResistantKlebsiella pneumoniae (MDR K. pneumoniae) is considered as an important opportunistic pathogen, which causes life threatening infections. K. pneumoniae is known to causes life-threatening infections. Objective: The objective of this study is to detect some β-lactamase genes in Klebsiella pneumoniae. Methods: Thirty-five K. pneumoniae isolates were obtained from some hospitals in Baghdad City between October 2022 and February 2023. The identification of K. pneumoniae isolates was done phenotypically by the automated VITEK II system and genotypically by amplification of the rpob gene. The antibiotic susceptibility and detection of some β-lactamase genes were tested for all isolates. Results: The results showed that the K. pneumoniae isolates were resistant to most antibiotics used. A high percentage of K. pneumoniae isolates were resistant to cefixime, cefpodoxime, norfloxacin and doxycycline as 100% followed by ceftriaxone, ceftazidime, ticarcillin/clavulanate, ticarcillin, ceftazidime, tobramycin, and moxifloxacin (97.2%, 91.7%, 91.7%, 94.4%, 94.4%, and 94.4%, respectively). Furthermore, approximately 94.4% of the isolates were MDR. In addition, the molecular methods only detected the blaNDM and blaTEM genes as 33.3% and 75.6% of in the K. pneumoniae isolates, respectively, while the blaVIM, blaCTX−M, and blaKPC genes were not observed in K. pneumoniae isolates. Conclusions: β-lactam antibiotics, including carbapenems, are widely used to treat bacterial infections; however, an increase in antibiotic resistance due to β-lactamases limits the effectiveness of these antibiotics. Therefore, alternative treatment methods are required to control these resistant isolates.
- Subjects
KLEBSIELLA pneumoniae; GENE amplification; TOBRAMYCIN; NORFLOXACIN; CEFTAZIDIME; BETA-lactamase inhibitors
- Publication
Al-Mustansiriyah Journal of Science, 2024, Vol 35, Issue 2, p52
- ISSN
1814-635X
- Publication type
Article
- DOI
10.23851/mjs.v35i2.1472