We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
牛源乳腺致病性大肠杆菌JL05全基因测序 及毒力和耐药基因分析.
- Authors
曲星霖; 肖 丹; 吴 健; 马晓媛; 白 翠; 王 妍; 王 楠
- Abstract
The aim of this study was to analyze the phylogenetic groups, virulence factor distribution, and the correlation between drug resistance phenotype and resistance genes of Escherichia coli JL05 isolated from milk samples of dairy cows. The PCR, microdilution method and high-throughput sequencing were used to investigate the phylogenetic groups, MIC of 16 antibacterial drugs, virulence genes and resistance genes. PCR results showed that E. coli JL05 belonged to group B2. MIC results showed that E. coli JL05 was sensitive to sulfamethoxazole and nitrofurantoin, but resistant to 14 of Class 8 antibacterial drugs, including β-lactam, cephalosporin, aminoglycoside, folic acid, quinolone, macrolide, tetracycline and phenylpropanol. The results of whole-genome sequencing showed that E. coli JL05 had fec locus which is specific to mammary pathogenic E. coli (MPEC). The analysis of the genome through VFDB database showed that E. coli JL05 had 80 virulence genes in six categories, including adhesion, invasion, transport, iron uptake, secretion system and bacterial toxin. In particular, Yersinia high pathogenicity island (HPI) was also detected. The analysis of the genome through CARD database showed that E. coli JL05 had 53 resistance genes involved in antibiotic efflux, antibiotic inactivation, change of antibiotic target and reduction of antibiotic permeability; and multiple trans-posase and integrase genes were found in the upstream and downstream of the plasmid resistance genes. This study revealed the complex evolutionary history of E. coli JL05, and provided a reference for a more comprehensive understanding of genomic characteristics, pathogenicity and drug resistance transmission mechanism of E. coli JL05.
- Publication
Chinese Journal of Preventive Veterinary Medicine / Zhongguo Yufang Shouyi Xuebao, 2020, Vol 42, Issue 12, p1220
- ISSN
1008-0589
- Publication type
Article
- DOI
10.3969/j.issn.1008-0589.202010002