We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Investigation of the Anti-cataract and Antioxidant Activities of Cnidoscolus aconitifolius Leaves Extract In vitro.
- Authors
Bulama, Henry Gideon; Dahiru, Daniel; Madu, Joshua Osuigwe
- Abstract
Background: Cataract is a major cause of visual impairment and blindness around the world. This study evaluated the in vitro antioxidant and anti-cataract activities of Cnidoscolus aconitifolius leaves extract and fractions. Antioxidant activities were evaluated by 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2-azinobis (3- ethylbenzothiozoline-6-sulfonic acid) (ABTS), total reducing power, and hydrogen peroxide scavenging assays. Anti-cataract potential was evaluated in vitro using goat lenses divided into eight groups of different treatments and incubated in artificial aqueous humor at 37 °C for 72 hours. Glucose-induced opacity in the lenses was observed and biochemical indices quantified (catalase, Malondialdehyde (MDA) and total protein in the lens homogenate). Results: The crude extract and its fractions possess substantial antioxidant activities. The aqueous fraction exhibited the best DPPH radical scavenging activity (IC50 value 78.599 μg/ml); while the dichloromethane fraction exhibited the highest ABTS radical scavenging activity with IC50 66.68 μg/ml. The anti-cataract evaluation of crude and fractions at 250 μg/ml showed a significant increase (p<0.05) in the total protein and catalase activity compared to the cataract control group. The malonaldehyde level decreased significantly (p<0.05) in all the treated groups. Conclusion: These results suggest that Cnidoscolus aconitifolius leaves extract possesses protective actions against oxidative damage and cataract caused by oxidative stress.
- Subjects
CATARACT; CRYSTALLINE lens diseases; ANTIOXIDANTS; CNIDOSCOLUS; SULFONIC acids
- Publication
Iraqi Journal of Science, 2021, Vol 62, Issue 1, p28
- ISSN
0067-2904
- Publication type
Article
- DOI
10.24996/ijs.2021.62.1.3